Bacteria Contaminants Associated With Poultry Feeds from Three Different Companies

Bacteria Contaminants Associated With Poultry Feeds from Three Different Companies

Literature Review

Among raw food items of animal origin, poultry meat and poultry meat products are considered to be some of the most contaminated products offered to the consumer (Klinger, 1993).  This is due to the relatively high contamination with various food-borne infection agents present on and in the product.  More than 27 different species of bacteria have been isolated from normal, ready to cook, poultry meat (cox and Bailey, 1987).  A large number of publications incriminate poultry as origin of food – borne infection out-breaks

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Modern production facilities for poultry tend to be large and intensive.  A very large number of birds are maintained in each production unit.  Under these conditions, it is nearly impossible to present the spread of a microorganism, once it has measures to control contamination of poultry with pathogenic Microorganisms (such as salmonella, listeria, and campylobacter) are aimed, inter-alia, towards prevention of the infection or colonization of the birds on the farm by various means of inhibiting possible entrances of disease producing agents into the farm.  High priority is given to decontamination of poultry feed, since all means employed to prevent infection will fail unless a supply of decontaminated feed is ensured.

The role of animal feed as a Vector of pathogenic microorganisms, in particular salmonella has been well documented ins the literature of the last decades, (Klinger and Lapidot, 1993).  Feed ingredients of animals origin, such as fishmeal, meat and bone meats, slaughter ofals and feather meals are reported to be usually contaminated.  However, ingredients of plant origin, such as oil-seed lake meals, are also reported to be contaminated, albeit at a relatively lower frequency (WHO, 1993).  In sevel countries, compulsory use of decontaminated feed is an integrat part of salmonella control in poultry in france, sweden, U.S.A (WHO, 1992).

In the report of an expert group on animal feed stuffs in the U.K.,it was stated that:  The three major potential sources of infection in poultry flocks are parent birds, the environment and feeding stuffs.  Clearly, the breeding bird can be a source of initial infection, is unclear.  In addition, the environment may become heavily contaminated and may be a cause in further transmission.  However, it is difficult in the current salmonella enteritis outbreak to assess the contribution of feeding stuffs relative to other sources at the present time. this appears to be a well established zoonosis in which an infection, possibly introduced by feeding stuffs, has resulted in further vertical transmission and widespread environmental contamination (Lamming, 1992).

The poultry industry, more than that of any other farm animal branch, depends largely on industrially produced feed constituting the sole diet of the birds. Decontamination of the feed is therefore, essential and is an integral part of the hygienic chain food manufacturing practice.  Methods developed for this purpose are based mainly on thermal treatment, usually employed ins the form of pelleting (Combination of humid heat and pressure) (WO, 1989 and 1990).  This treatment is effective only if a sufficiently high temperature is employed for enough time to eliminate the pathogens completely and if the pellets are protected during cooling from recontamination by employing filtered air for prevention of dust and by preventing condensation.

Chemical treatment, utilizing organic acids such as tonic or propionic acid, is also common, both with good results (WHO, 1989 and 1990).  This is effective under the condition that a sufficiently highly concentration of acids or their derivatives is applied (often too costly for the poultry industry) and that sufficient time is available for their diffusion into the feed grannles.  However, their use is accompanied by corrosion of equipment as well as by environmental hazards, requiring protective face and body gear for the personnel involved.

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Limitations imposed on the feed industry will affect the decision on the method to be employed.  The method should be effective, safe form a spoilt of view of public health, performance and quality of the products, as well as for the health of consumers.  And all  this at the lowest cost possible.  The economic aspect is of immense importance since the poultry industry is calculating its profits in terms of very small increments.

One proven alternative method for decontamination of ionizing radiation (Campbell et al, 1986, coming, 1983; Ho et al; 1979, Klinger and Lapidot, 1993; Kocher, 1991; Lay et al, 1969 and Mossed, 1979)>  This method presents several advantages.  The major advantages  are that the method can be applied to products in their final commercial package or that it can be  applied on line, using accelerators, to continously flowing, comminnted feed or feed components at very high throughputs, and at relatively low costs. Recent calculations shows that iorradiation treatment of poultry feed could increase the price of the feed by 3% and that of the broiler meat  istself, by 0.5 1% (WHO, 1991), one should note, however, that when irradiated with accelerated elecytrons, in  free flow, precautions are needed to prevent recontamination.

Although feed is not food, it is declined practical (in some countries) to adopt food standards for a feed which is co directly linked to the final quality of the product as in the case of poultry and its feed.

Poultry feed ingredients are delivered in bulk and usually in very large quantities, conveyed directly from one site to another.  The poultry industry relies on a supply of ready – to – use feed from a feed mill, constructed for handling very large quantities of different ingredients, including unloading, grinding of grains, mixing and usually pelleting of the mixed ration.  All ingredients, excluding those added in micro-amounts, are stored in sibs, from which the materials are conveyed to the mixing facilities in accordance with compatrized control of mixed ration.  If the ingredients are irradiated prior to recontamination by special from recontamination by special measures, such as filtered air, to exclude dust and insects.

Different conditions prevail in facilities for small-scale poultry farms, where the packaging material s used for feed and or feed components are sacks made of plastic, laminated paper or gute.  If such ingredients are to be irradiated in their original packaging, the following should be  considered: (a) generally, at the dose required, commonly used packaging materials are satisfactory for irradiated products; (b) packing should be functionally proteive, (c ) where irraadiation significantly alters the funcitonal properties to a particular packaging material, or may result iin the formation of toxic substances which can be transffered by contact to the feed component, that packaging materials cannot be used.  The choice of packaging materials may be restricted by regulation in the country where the product is produced and or sold. Pakaging materials used must comply with relevant national and local regulations.

Poultry feed components of plant and animal origin are commonly contaminated with microorganisms (Bacteria  and moulds) and or insects. S the numbers and types of microorganisms and insects vary with the particular material the location of its origin, climatic conditions, encountered, harvesting, communiting, processing, storage and transport technologies employed, packaging used, and the general environmental and handling circumstances, including the nature and extent of quality control measures.

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Elimination of Salmonella and of other enteric bacterial pathogens, such as particular types of Escherichia coli, and listeria monocytogens from feed components, can be achieve at relatively low absorbed doses.  Micro-organisms belonging to the Enterobacteriaceae family are generally radiation susceptible and can generally be eliminbated.  (Begun et al, 1989; l fonly et al, 1986) Ho et al, 1981; Lapidot et al 1968).

The number of moulds, such as Aspergillus, pencillium, Fusarium spp.  Is often reduced significantly at an absorbed dose of 5kGy. However, mycotoxins produced before the treatment are not significantly affected by radiation (El far et al, 1992; Kuline et al, 1983; Odaintten et al, 1980 and Radova et al, 1991).  From the practical point of view, the absorbed dose of 3-7 kGy at the point of minimum dose rate withoin the product, s sufficient to decrease the number of microorganisms to an acceptable level, without causing significant chemical changes in feeds (Adler et al, 1978).  It is known that lipids are attacked by free radicals which are formed upon irradiation and that peroxides and other oxidation products are formed under aerobic conditions in a way similar to the autoxidation process.  However, farm animal did treated with ionizing radiation are likely to be acceptable to the animal because irradiation at 6kGy forms only small quantities of oxidation products of fat in the feeds (Lapidot, 1979).  It should be noted that effective absorbed doses required for greater than those needed for insects.  Therefore, irradiation of poultry feed components for control of the microflora will completely eliminate any insect present, at all stages of development (Lorenz, 19978).

It should also be noted that, if feeds or feed components previously decontaminated by heat treatment (Pelletization) or by other means (rganic acids), have been reconstaminated during cooling, handling and storage (usually at a low level), the radiation dose required for elimination of the added pathogens may be quite low and should be determined as a function of the residual counts.  The fact that feeds or feed components have been irradiated should where required, be specified in proper documentation,  using expressions such as “irradiated” or treated with ionizing radiation” with indication of the purpose, e.g. disinfestation or decontamination.  Thus the feed or feed component is not only to be identified as is also to be informed as to the  purpose and benefits of the treatment (Eisenberg and Lapidot, 1978).

The purpose of processing feed grain is to improve their palatability, digestibility and overall feed efficiency.  For many years, poultry stockmen sought to do this by grinding, crush  rolling and soaking feed grains but since the late  50’s several new processing techniques have been used (McGraw – Hill encyclopaedia, 1992). Grinding and pelleting are the most common means of preparing feed for poultry (Leonard, 1981).  Generally, results shore that grinding to a maximum moderately fine texture results in bet performance than when grains are finely grind (Church, 1988). Feed particles of different ingredients should be of since size so that birds do not sort  on the coarse particles and leave the fine particles. Pelleting usually results in improved grain, partly because animals tend to eat more in a give period.  Efficiency often is improved, sometimes because of less wastage with pellets.

CHAPTER THREE

  • MATERIALS AND METHODS

3.1     MATERIALS (SEE APPENDIX)

3.2     METHODS

  • STERILIZATION

Already, properly washed test tubes, petri-dishes, pipettes, conical flasks, beakers, bottles, wire loop, spatulas and inoculating needles, were sterilized in hot air oven at 160c for one hour in their respective canisters and stored at 41c.

3.2.2  SOURCE OF SAMPLES

The type of poultry feeds used in the research were starters mash from three different companies (i.e Top feed, vital and Guinea feed).  The starters mash were collected with sterile spatulas from 150 different bags in the ratio of 50¨500 and were promptly transferred to the laboratory for analysis

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3.2.3  PREPARATION OF CULTURE MEDIA

All the media used for culturing were aseptically preapred accoring to the manufacturers instructions and autoclaved at 121c for 15 mins at 15165/Ps1.  The media include nutrient agar and MacConkey

agar.

  • DETERMINATION OF pH

The pH was determined using the method described by Ofunya and Akpoti (1988). Lg sample of poultry feed was suspended into 30ml of demonized neater.  It was stirred and allowed to settle for 1 minutes before the pH was determined with a pH meter (Table 3).

3.2.5           PLATING TECHNIQUE

The “streak plate” method was employed. A loopful of suspension was removed aseptically, from each of the samples, using a sterile wire loop. Then the loop was streaked very gently over the surface of the solidified Nutrient agar.  Care was taken so that there was no cut into the agar with the wire loop.  The streaking was done in a characteristic patterns, in which the  loop was drawn over a surface of the agar with three streaks.  The loop was flamed another three parallel streaks made over area B and latter C area, and completed with a snake – like streak towards the centre of the plate.  The plates were covered, inverted and incubate for 24 hours at 37c

3.2.6  BACTERIA COUNT, GRAM STAINING AND MICROSCOPIC:-

  1. BACTERIA COUNT:-

After incubation the number of colonies on the petri-dishes were counted using the colony counting chamber.  The average total and differential standard plate counts (SPC) were taken (Table 1 and 2 respectively).

  1. GRAM STAINING AND MICROSCOPIC WORK:

Grown colonies were gram-stained using representatives of the different groups. Later the stained smears were examined microscopically.  Later representative colonies were separately sub-cultured for confirmatory tests.

3.2.7  BIOCHEMICAL TEST FOR IDENTIFICATION OF BACTERIAL ISOLATES.

A       CATALASE TEST

This test was performed according to the method of Barker and silverton (1985). With the aid of a glass rod, a portion of the bacterial colony was transferred on to a clean slide.  Two drops of hydrogen peroxide were placed on the organism and left for a few minutes. Production of gas bubbles indicated a positive reaction.

B.      INDOLE TEST

The peptone water medium was inoculated and incubated for 48 hours at 37c.  the tube were further allowed to stay for more 48 hours in the incubator for the accumulation of indole.  After this period,  0.5ml kova’s reagent was added separately to each tube and swirled gently.  The appearance of a red colour in the alcohol layer indicated a positive reaction.

Bacteria Contaminants Associated With Poultry Feeds from Three Different Companies

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